Working under direct supervision and report to Dr. Geoff de Couto, PhD., as a Research Associate I, you will perform a variety of routine experimental protocols and procedures to support the objectives of one or more laboratory research projects in a specific area of research. Assists in general laboratory activities, including maintenance of reagents and stocking of supplies. Files and monitors documents. Keeps accurate and detailed records of experiments and results. Assists in the operation of specialized equipment and machinery. Observes safety standards and procedures.
You will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining. This position does not have supervisory responsibilities.
Additional expectations include:
Performing a variety of routine laboratory tasks and procedures.
Keep accurate and detailed project records of experiments and results.
May be asked to assist with animal husbandry.
Maintain lab equipment and related records.
Transport, process and log samples.
Maintains computer database with relevant clinical information.
Perform lab maintenance duties, including glassware cleaning and sterilization.
Maintain stock of general lab materials, and places orders for lab equipment and supplies.
Assist new students and fellows at the laboratory.
Assist in the operation of specialized equipment and machinery.
Observe and comply with safety standards and procedures.
Department specific duties include:
Tissue harvest, embedding, and sectioning for downstream histological analyses.
Gene expression analyses (RNA extraction, cDNA synthesis, and qPCR) from cell culture and tissue samples.
Plasmid preparation (from bacterial culture to purification) and transfection.
Assist in the management of breeding pairs and experimental mouse colony to optimize animal availability for scheduled studies.
Assist in procuring material for mouse genotyping.
Perform echocardiographic image acquisition of aortic dimensions and cardiac function.
Assist in the analysis and quantification of echocardiography images for quantification of aortic and cardiovascular variables of interest.
Perform drug delivery via sub-cutaneous, intraperitoneal, gavage, or surgically installed osmotic minipumps.
Perform or assist in the sacrifice of mice, and procurement of tissues for (1) ex vivo phenotype assessments such as seahorse mitochondrial metabolism assay and aortic ring myography; (2) fixation or snap-freezing of tissues for molecular analyses and immunostaining; (3) preparation of cultured cell lines from vascular tissue beds.
Assist in western blotting, immunostaining, and physiology experiments downstream of mouse drug studies as available.
Providing healthcare for more than 100 years, Cedars-Sinai has evolved into one of the most dynamic and highly renowned medical centers in the world. Along with caring for patients, Cedars-Sinai is a hub for biomedical research and a training center for future physicians and other healthcare professionals. This attracts exceptional talent to Cedars-Sinai, including world-renowned physician-scientists who seek a place where they can both conduct research and see patients--the ideal formula for discovery and its translation into cures. Our patients benefit from access to doctors at the top of their fields, and our researchers have an ideal community in which to study the impact of healthcare challenges, and reflect that knowledge in their research. The greater Los Angeles area in which Cedars-Sinai resides possesses unparalleled cultural and ethnic diversity which offers outstanding opportunities for translational and clinical research... and a dynamic environment for medical education.Although community based, Cedars-Sinai is a major teaching hospital affiliated with the David Geffen School of Medicine at the University of California, Los Angeles (UCLA). Cedars-Sinai has highly competitive graduate medical education programs in more than 50 specialty and subspecialty areas, a graduate program in biomedical sciences and translational medicine, a clinical scholars program directed towards junior physicians with aspirations to become clinical scientists, and post graduate training opportunities.There are more than 250 full-time faculty members at Cedars-Sinai. The voluntary medical staff, comprised of more than 2,200 specialty board-certified or board-qualified physicians, represent all of the specialties and subspecialties and collaborate with full-time medical staff in the teaching responsibilities of the graduate medical education programs.