The Merchant lab applies cutting edge immune profiling modalities to study the immune system in patients with hematologic malignancies. In particular, we are interested in understanding the function of NK and T cell biology in the context of allogeneic bone marrow transplant, adoptive cell transfer, CART cells, bi-specific immune cell engagers (BiTE) and oncolytic virus therapy. Our lab runs the imaging mass cytometry/CyTOF core facility at Cedars-Sinai. Imaging mass cytometry allows for highly multiplexed (35+ markers) imaging of immune and tumor cells, with spatial information and subcellular resolution. Imaging mass cytometry can be applied to all tissue types including cell lines, organoid, frozen section and FFPE fixed archival tissue. As a Research Associate I in the Merchant lab, you would assist with performing imaging mass cytometry and suspension mass cytometry.
Primary Duties and Responsibilities:
Transports, processes, and logs samples for mass cytometry analysis.
Performs staining of tissue or suspension samples for mass cytometry.
Performs conjugations and validation of antibodies and other reagents used for mass cytometry work.
Maintains lab equipment and related records and may coordinate use of lab equipment.
Maintains computer database with relevant information.
Performs lab maintenance duties, including glassware cleaning and sterilization.
Maintains stocks of general lab materials, and places orders for lab equipment and supplies.
Assists new students and fellows at the laboratory.
Assists in the operation of specialized equipment and machinery including mass cytometry system
Observes and complies with safety standards and procedures.
Keeps accurate and detailed project records of experiments and results.
Follows, updates and occasionally prepares laboratory SOP
Providing healthcare for more than 100 years, Cedars-Sinai has evolved into one of the most dynamic and highly renowned medical centers in the world. Along with caring for patients, Cedars-Sinai is a hub for biomedical research and a training center for future physicians and other healthcare professionals. This attracts exceptional talent to Cedars-Sinai, including world-renowned physician-scientists who seek a place where they can both conduct research and see patients--the ideal formula for discovery and its translation into cures. Our patients benefit from access to doctors at the top of their fields, and our researchers have an ideal community in which to study the impact of healthcare challenges, and reflect that knowledge in their research. The greater Los Angeles area in which Cedars-Sinai resides possesses unparalleled cultural and ethnic diversity which offers outstanding opportunities for translational and clinical research... and a dynamic environment for medical education.Although community based, Cedars-Sinai is a major teaching hospital affiliated with the David Geffen School of Medicine at the University of California, Los Angeles (UCLA). Cedars-Sinai has highly competitive graduate medical education programs in more than 50 specialty and subspecialty areas, a graduate program in biomedical sciences and translational medicine, a clinical scholars program directed towards junior physicians with aspirations to become clinical scientists, and post graduate training opportunities.There are more than 250 full-time faculty members at Cedars-Sinai. The voluntary medical staff, comprised of more than 2,200 specialty board-certified or board-qualified physicians, represent all of the specialties and subspecialties and collaborate with full-time medical staff in the teaching responsibilities of the graduate medical education programs.