Research - Laboratory/Non-Laboratory, Staff/Administrative
UCSD Layoff from Career Appointment: Apply by 02/06/19 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 2/19/19. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The multi-disciplinary laboratory within the Department of Pediatrics works in the fields of RNA and Chemical biology. The laboratory explores the role of non-coding RNAs in stem cell biology and host-pathogen interactions. Projects in the lab use cell culture, animal models, neural stem cells, 3D organoids, human pluripotent cells and several molecular tools to investigate fundamental mechanisms injury, AIDS, and immune disorders.
Under general supervision of the Principal Investigator, the Staff Research Associate III will perform cellular lab experiments, making significant independent contributions in the design, troubleshooting, modification and analysis of experiments and protocols. Incumbent will also perform experiments using tissue culture techniques such as PCR, plasmid purification, iPSC production and characterization, and HT screening and sequencing, gene silencing, cloning, CRISPR genome editing, under general supervision.
In addition, the incumbent will independently support duties to include RT-PCR, qPCR, DNA and RNA molecular experiments, western blotting, immunoprecipitation, affinity purification, analyze and report data, prepare complex solutions, reagents and culture media and run cell analyses to ensure quality and control.
The incumbent will be responsible for implementing and performing a variety of new and on-going experimental protocols, making significant research contributions to the laboratory and making innovative contributions pertaining to complex experimental procedures of iPSC production and characterization. May provide training and direction and work cooperatively with other SRAs, including doctoral and student lab assistants. Compile and analyze research data, draft articles and grant proposals.
Advanced theoretical knowledge of and direct experience with Stem Cell biology as typically attained by a Bachelor's Degree; or an equivalent combination of education and experience.
Strong experience with research design, protocol development, and qualitative and quantitative research methods to independently address scientific challenges to move the project forward.
Demonstrated direct experience overseeing research projects.
Proven ability to perform scientific procedures including confocal microscopy, DNA and RNA isolation, and histological analysis.
Proven experience authoring journal articles, abstracts, and posters. Ability to prepare papers for publication and research proposals.
Ability to read, comprehend and discuss research materials. Review scientific articles and make innovative contributions to develop new research techniques and improve current protocols for the research projects.
Demonstrated knowledge of microscopy imaging procedures and in vivo analysis.
Demonstrated experience in analysis and management of complex data.
Demonstrated experience in qualitative research methods.
Advanced knowledge and experience working with and handling small animals including mice. Knowledge of normal physiological data of rodents, particularly mice.
Experience with small animal husbandry and excellent organizational skills to manage all lab animal work.
PhD in cell biology, biochemistry, chemical biology, or genetics.
Employment is subject to a criminal background check.
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